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Frequently asked questions (FAQs)

How can I tell if my oligos successfully annealed?

Nov 17, 2016, 18:17 PM
Question : How can I tell if my oligos successfully annealed?

The hybridized oligos or DNA duplex can be run on a non-denaturing gel with appropriate molecular weight markers.

Alternatively, run the duplexed oligo side by side with a single-stranded oligo. If annealing was successful, the double-stranded band will be shifted up from the single-stranded band.

Use UV back shadowing or a stain such as SYBR™ Gold to visualize the bands. Ethidium bromide may not sufficiently stain the single-stranded DNA to make it visible.

Categories :
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Tags :
  • dna synthesis
  • oligonucleotide
  • pcr
  • primers
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