qPCR & PCR articles
Annealing temperature FAQs
Using the best annealing temperature is important for optimal hybridization and amplification and to avoid annealing to sequences other than the intended target. Review these FAQs for tips and tricks for finding the optimal annealing temperature for your oligos.
- Annealing temperature FAQs
- How to design qPCR primers that span an exon junction
- Primer design and other tools you should know about
- Colony PCR: What is it and why is it important
- PrimeTime One-Step 4X Broad-Range Master Mix for one-step RT-qPCR experiments
- Recommended dye combinations for multiplex qPCR
- A Quick Guide for troubleshooting qPCR data
- SUN™ fluorophore
- Improve your assays with customizable oligos and probes containing cost-effective locked nucleic acids
- Genotyping: Terms to know
- Consider SNPs when designing PCR and qPCR assays
- A qPCR master mix that can be shipped at ambient temperatures
- Obtain high quality qPCR results using PrimeTime™ Gene Expression Master Mix
- Primer design tools for PCR & qPCR
- Could your PCR be affected by contamination?
- qPCR probes—Selecting the best reporter dye and quencher
- Explaining multiple peaks in qPCR melt curve analysis
- A fast, cost-effective alternative to radiolabeling and HPLC/MS for measuring dNTPs
- How to design primers and probes for PCR and qPCR
- Double-quenched probes increase signal to noise ratios by decreasing background fluorescence
- Designing standard curves for qPCR
- Is GAPDH a good reference sequence?
- How to avoid false positives in PCR and what to do if you get them
- DNA methylation analysis—Keeping it simple
- qPCR (Real-Time PCR): Methods, Types, Controls
- Determining the physical characteristics of your oligos
- Steps for a successful qPCR experiment
- Starting with RNA—One‑step or two‑step RT‑qPCR?
- Using BLAST to find primers—Tips from IDT
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